Construct: shRNA TRCN0000201582

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033079.1-1038s1c1
DNA Barcode:
CCTTCATTCTCAGACCCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
M1ap (110958)
Gene Description:
meiosis 1 associated protein
Transcript:
RefSeq NM_033079.1 (NON-CURRENT)
Match location:
Position 1038 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201582)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCATTCTCAGACCCACAA
Hairpin Sequence:
5'-CCGG-CCTTCATTCTCAGACCCACAA-CTCGAG-TTGTGGGTCTGAGAATGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCATTCTCAGACCCACAACTCGAGTTGTGGGTCTGAGAATGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCATTCTCAGACCCACAACTCGAGTTGTGGGTCTGAGAATGAAGG-3'

Other clones with same target sequence:

(none)