Construct: shRNA TRCN0000201666

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178264.2-473s1c1
DNA Barcode:
GAGAACAACAGAGAAGCCTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D830050J10Rik (352968)
Gene Description:
RIKEN cDNA D830050J10 gene
Transcript:
RefSeq NM_178264.2 (NON-CURRENT)
Match location:
Position 473 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201666)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAACAACAGAGAAGCCTTC
Hairpin Sequence:
5'-CCGG-GAGAACAACAGAGAAGCCTTC-CTCGAG-GAAGGCTTCTCTGTTGTTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAACAACAGAGAAGCCTTCCTCGAGGAAGGCTTCTCTGTTGTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAACAACAGAGAAGCCTTCCTCGAGGAAGGCTTCTCTGTTGTTCTC-3'

Other clones with same target sequence:

(none)