Construct: shRNA TRCN0000201696

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181594.2-2947s1c1
DNA Barcode:
GCACTAATTTGGCAGCAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Edc4 (234699)
Gene Description:
enhancer of mRNA decapping 4
Transcript:
RefSeq NM_181594.2 (NON-CURRENT)
Match location:
Position 2947 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201696)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTAATTTGGCAGCAGCAA
Hairpin Sequence:
5'-CCGG-GCACTAATTTGGCAGCAGCAA-CTCGAG-TTGCTGCTGCCAAATTAGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTAATTTGGCAGCAGCAACTCGAGTTGCTGCTGCCAAATTAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTAATTTGGCAGCAGCAACTCGAGTTGCTGCTGCCAAATTAGTGC-3'

Other clones with same target sequence:

(none)