Construct: shRNA TRCN0000201734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024614.1-240s1c1
DNA Barcode:
GCGAGAAAGGCAGCTATCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1700007G11Rik (75784)
Gene Description:
RIKEN cDNA 1700007G11 gene
Transcript:
RefSeq NM_001024614.1 (CURRENT)
Match location:
Position 240 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGAAAGGCAGCTATCAAT
Hairpin Sequence:
5'-CCGG-GCGAGAAAGGCAGCTATCAAT-CTCGAG-ATTGATAGCTGCCTTTCTCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGAAAGGCAGCTATCAATCTCGAGATTGATAGCTGCCTTTCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGAAAGGCAGCTATCAATCTCGAGATTGATAGCTGCCTTTCTCGC-3'

Other clones with same target sequence:

(none)