Construct: shRNA TRCN0000201761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026739.1-187s1c1
DNA Barcode:
GCCTGAACATGAACTTGCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
9530077C05Rik (68283)
Gene Description:
RIKEN cDNA 9530077C05 gene
Transcript:
RefSeq NM_026739.1 (CURRENT)
Match location:
Position 187 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGAACATGAACTTGCTAA
Hairpin Sequence:
5'-CCGG-GCCTGAACATGAACTTGCTAA-CTCGAG-TTAGCAAGTTCATGTTCAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGAACATGAACTTGCTAACTCGAGTTAGCAAGTTCATGTTCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGAACATGAACTTGCTAACTCGAGTTAGCAAGTTCATGTTCAGGC-3'

Other clones with same target sequence:

(none)