Construct: shRNA TRCN0000201995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144866.2-1572s1c1
DNA Barcode:
CCGTGAGCATTGGATACTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Etf1 (225363)
Gene Description:
eukaryotic translation termination factor 1
Transcript:
RefSeq NM_144866.2 (NON-CURRENT)
Match location:
Position 1572 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000201995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGAGCATTGGATACTGAA
Hairpin Sequence:
5'-CCGG-CCGTGAGCATTGGATACTGAA-CTCGAG-TTCAGTATCCAATGCTCACGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGAGCATTGGATACTGAACTCGAGTTCAGTATCCAATGCTCACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGAGCATTGGATACTGAACTCGAGTTCAGTATCCAATGCTCACGG-3'

Other clones with same target sequence:

  1. TRCN0000279073