Construct: shRNA TRCN0000202067

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145605.1-1849s1c1
DNA Barcode:
CGCAATGGTTTCACCATCACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klhdc4 (234825)
Gene Description:
kelch domain containing 4
Transcript:
RefSeq NM_145605.1 (NON-CURRENT)
Match location:
Position 1849 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202067)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAATGGTTTCACCATCACT
Hairpin Sequence:
5'-CCGG-CGCAATGGTTTCACCATCACT-CTCGAG-AGTGATGGTGAAACCATTGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAATGGTTTCACCATCACTCTCGAGAGTGATGGTGAAACCATTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAATGGTTTCACCATCACTCTCGAGAGTGATGGTGAAACCATTGCG-3'

Other clones with same target sequence:

(none)