Construct: shRNA TRCN0000202128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029394.2-451s1c1
DNA Barcode:
CCTCTCTACCGAAAGCAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snx24 (69226)
Gene Description:
sorting nexing 24
Transcript:
RefSeq NM_029394.2 (NON-CURRENT)
Match location:
Position 451 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCTACCGAAAGCAGAAA
Hairpin Sequence:
5'-CCGG-CCTCTCTACCGAAAGCAGAAA-CTCGAG-TTTCTGCTTTCGGTAGAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCTACCGAAAGCAGAAACTCGAGTTTCTGCTTTCGGTAGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCTACCGAAAGCAGAAACTCGAGTTTCTGCTTTCGGTAGAGAGG-3'

Other clones with same target sequence:

(none)