Construct: shRNA TRCN0000202133

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178248.2-979s1c1
DNA Barcode:
CCAAGCATCCATCAGCTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pramel4 (347709)
Gene Description:
preferentially expressed antigen in melanoma like 4
Transcript:
RefSeq NM_178248.2 (NON-CURRENT)
Match location:
Position 979 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202133)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGCATCCATCAGCTCAAA
Hairpin Sequence:
5'-CCGG-CCAAGCATCCATCAGCTCAAA-CTCGAG-TTTGAGCTGATGGATGCTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGCATCCATCAGCTCAAACTCGAGTTTGAGCTGATGGATGCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGCATCCATCAGCTCAAACTCGAGTTTGAGCTGATGGATGCTTGG-3'

Other clones with same target sequence:

(none)