Construct: shRNA TRCN0000202167
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_146219.1-1239s1c1
- DNA Barcode:
- CCGCCTCCAACCTTCTTTATA
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Klhl36 (234796)
- Gene Description:
- kelch-like 36
- Transcript:
- RefSeq NM_146219.1 (CURRENT)
- Match location:
- Position 1239 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000202167)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- CCGCCTCCAACCTTCTTTATA
- Hairpin Sequence:
- 5'-CCGG-CCGCCTCCAACCTTCTTTATA-CTCGAG-TATAAAGAAGGTTGGAGGCGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCGCCTCCAACCTTCTTTATACTCGAGTATAAAGAAGGTTGGAGGCGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCGCCTCCAACCTTCTTTATACTCGAGTATAAAGAAGGTTGGAGGCGG-3'