Construct: shRNA TRCN0000202207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175280.2-791s1c1
DNA Barcode:
CCCTCCTTTCTACTTCCTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfap61 (78774)
Gene Description:
cilia and flagella associated protein 61
Transcript:
RefSeq NM_175280.2 (NON-CURRENT)
Match location:
Position 791 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCTTTCTACTTCCTGTT
Hairpin Sequence:
5'-CCGG-CCCTCCTTTCTACTTCCTGTT-CTCGAG-AACAGGAAGTAGAAAGGAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCTTTCTACTTCCTGTTCTCGAGAACAGGAAGTAGAAAGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCTTTCTACTTCCTGTTCTCGAGAACAGGAAGTAGAAAGGAGGG-3'

Other clones with same target sequence:

(none)