Construct: shRNA TRCN0000202290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026909.1-907s1c1
DNA Barcode:
GAAGGACCATGTACAGGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thap7 (69009)
Gene Description:
THAP domain containing 7
Transcript:
RefSeq NM_026909.1 (NON-CURRENT)
Match location:
Position 907 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGACCATGTACAGGACTT
Hairpin Sequence:
5'-CCGG-GAAGGACCATGTACAGGACTT-CTCGAG-AAGTCCTGTACATGGTCCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGACCATGTACAGGACTTCTCGAGAAGTCCTGTACATGGTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGACCATGTACAGGACTTCTCGAGAAGTCCTGTACATGGTCCTTC-3'

Other clones with same target sequence:

  1. TRCN0000277337