Construct: shRNA TRCN0000202325

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182808.1-276s1c1
DNA Barcode:
GTGGATAAGTGCTTGTGCGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam19a1 (320265)
Gene Description:
family with sequence similarity 19, member A1
Transcript:
RefSeq NM_182808.1 (NON-CURRENT)
Match location:
Position 276 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202325)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGATAAGTGCTTGTGCGAT
Hairpin Sequence:
5'-CCGG-GTGGATAAGTGCTTGTGCGAT-CTCGAG-ATCGCACAAGCACTTATCCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGATAAGTGCTTGTGCGATCTCGAGATCGCACAAGCACTTATCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGATAAGTGCTTGTGCGATCTCGAGATCGCACAAGCACTTATCCAC-3'

Other clones with same target sequence:

(none)