Construct: shRNA TRCN0000202489

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029939.1-1921s1c1
DNA Barcode:
GCAATAGGTTGCGCAGATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc151 (77609)
Gene Description:
coiled-coil domain containing 151
Transcript:
RefSeq NM_029939.1 (NON-CURRENT)
Match location:
Position 1921 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202489)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATAGGTTGCGCAGATCTT
Hairpin Sequence:
5'-CCGG-GCAATAGGTTGCGCAGATCTT-CTCGAG-AAGATCTGCGCAACCTATTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATAGGTTGCGCAGATCTTCTCGAGAAGATCTGCGCAACCTATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATAGGTTGCGCAGATCTTCTCGAGAAGATCTGCGCAACCTATTGC-3'

Other clones with same target sequence:

(none)