Construct: shRNA TRCN0000202546

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_128715.5-2019s1c1
DNA Barcode:
CAAAGTTAAAGAGTCTCGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Safb (224903)
Gene Description:
scaffold attachment factor B
Transcript:
RefSeq XM_128715.5 (NON-CURRENT)
Match location:
Position 2019 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202546)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGTTAAAGAGTCTCGAAA
Hairpin Sequence:
5'-CCGG-CAAAGTTAAAGAGTCTCGAAA-CTCGAG-TTTCGAGACTCTTTAACTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGTTAAAGAGTCTCGAAACTCGAGTTTCGAGACTCTTTAACTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGTTAAAGAGTCTCGAAACTCGAGTTTCGAGACTCTTTAACTTTG-3'

Other clones with same target sequence:

(none)