Construct: shRNA TRCN0000202739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_374422.1-1712s1c1
DNA Barcode:
CCCAAGAAGAACTTATAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPY19L1 (23333)
Gene Description:
dpy-19 like C-mannosyltransferase 1
Transcript:
RefSeq XM_374422.1 (NON-CURRENT)
Match location:
Position 1712 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGAAGAACTTATAGAAT
Hairpin Sequence:
5'-CCGG-CCCAAGAAGAACTTATAGAAT-CTCGAG-ATTCTATAAGTTCTTCTTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGAAGAACTTATAGAATCTCGAGATTCTATAAGTTCTTCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGAAGAACTTATAGAATCTCGAGATTCTATAAGTTCTTCTTGGG-3'

Other clones with same target sequence:

(none)