Construct: shRNA TRCN0000202931

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001011812.1-492s1c1
DNA Barcode:
CTTCATGTTTAGGATCCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr951 (258046)
Gene Description:
olfactory receptor 951
Transcript:
RefSeq NM_001011812.1 (CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000202931)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCATGTTTAGGATCCAGTT
Hairpin Sequence:
5'-CCGG-CTTCATGTTTAGGATCCAGTT-CTCGAG-AACTGGATCCTAAACATGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCATGTTTAGGATCCAGTTCTCGAGAACTGGATCCTAAACATGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCATGTTTAGGATCCAGTTCTCGAGAACTGGATCCTAAACATGAAG-3'

Other clones with same target sequence:

(none)