Construct: shRNA TRCN0000203211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_372596.1-611s1c1
DNA Barcode:
GATTTATTTCTCTGGCTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR4F6 (390648)
Gene Description:
olfactory receptor family 4 subfamily F member 6
Transcript:
RefSeq XM_372596.1 (NON-CURRENT)
Match location:
Position 611 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000203211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTTATTTCTCTGGCTTCTT
Hairpin Sequence:
5'-CCGG-GATTTATTTCTCTGGCTTCTT-CTCGAG-AAGAAGCCAGAGAAATAAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTTATTTCTCTGGCTTCTTCTCGAGAAGAAGCCAGAGAAATAAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTTATTTCTCTGGCTTCTTCTCGAGAAGAAGCCAGAGAAATAAATC-3'

Other clones with same target sequence:

(none)