Construct: shRNA TRCN0000203449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358273.1-379s1c1
DNA Barcode:
CCTTTCTTAAAGGACTGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC385534 (385534)
Gene Description:
similar to Spindlin-like protein 2 (SPIN-2)
Transcript:
RefSeq XM_358273.1 (NON-CURRENT)
Match location:
Position 379 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000203449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCTTAAAGGACTGGTTT
Hairpin Sequence:
5'-CCGG-CCTTTCTTAAAGGACTGGTTT-CTCGAG-AAACCAGTCCTTTAAGAAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCTTAAAGGACTGGTTTCTCGAGAAACCAGTCCTTTAAGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCTTAAAGGACTGGTTTCTCGAGAAACCAGTCCTTTAAGAAAGG-3'

Other clones with same target sequence:

(none)