Construct: shRNA TRCN0000203499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376713.1-626s1c1
DNA Barcode:
CCTTGTCCACAATTGTAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR2A7 (401427)
Gene Description:
olfactory receptor family 2 subfamily A member 7
Transcript:
RefSeq XM_376713.1 (NON-CURRENT)
Match location:
Position 626 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000203499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGTCCACAATTGTAGTTT
Hairpin Sequence:
5'-CCGG-CCTTGTCCACAATTGTAGTTT-CTCGAG-AAACTACAATTGTGGACAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGTCCACAATTGTAGTTTCTCGAGAAACTACAATTGTGGACAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGTCCACAATTGTAGTTTCTCGAGAAACTACAATTGTGGACAAGG-3'

Other clones with same target sequence:

(none)