Construct: shRNA TRCN0000203863

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_069595.4-294s1c1
DNA Barcode:
CGCTCAAATCTTCTTCATCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR4F16 (135896)
Gene Description:
olfactory receptor OR4F16
Transcript:
RefSeq XM_069595.4 (NON-CURRENT)
Match location:
Position 294 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000203863)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCAAATCTTCTTCATCCA
Hairpin Sequence:
5'-CCGG-CGCTCAAATCTTCTTCATCCA-CTCGAG-TGGATGAAGAAGATTTGAGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCAAATCTTCTTCATCCACTCGAGTGGATGAAGAAGATTTGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCAAATCTTCTTCATCCACTCGAGTGGATGAAGAAGATTTGAGCG-3'

Other clones with same target sequence:

(none)