Construct: shRNA TRCN0000204329

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172089.2-934s1c1
DNA Barcode:
CGCAGGTGTCTTCCATTTACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFSF12-TNFSF13 (407977)
Gene Description:
TNFSF12-TNFSF13 readthrough
Transcript:
RefSeq NM_172089.2 (NON-CURRENT)
Match location:
Position 934 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000204329)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGGTGTCTTCCATTTACA
Hairpin Sequence:
5'-CCGG-CGCAGGTGTCTTCCATTTACA-CTCGAG-TGTAAATGGAAGACACCTGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGGTGTCTTCCATTTACACTCGAGTGTAAATGGAAGACACCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGGTGTCTTCCATTTACACTCGAGTGTAAATGGAAGACACCTGCG-3'

Other clones with same target sequence:

  1. TRCN0000059026