Construct: shRNA TRCN0000204364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182487.1-972s1c1
DNA Barcode:
CGCCTTCACCAAGAACATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OLFML2A (169611)
Gene Description:
olfactomedin like 2A
Transcript:
RefSeq NM_182487.1 (NON-CURRENT)
Match location:
Position 972 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000204364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTTCACCAAGAACATCAT
Hairpin Sequence:
5'-CCGG-CGCCTTCACCAAGAACATCAT-CTCGAG-ATGATGTTCTTGGTGAAGGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTTCACCAAGAACATCATCTCGAGATGATGTTCTTGGTGAAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTTCACCAAGAACATCATCTCGAGATGATGTTCTTGGTGAAGGCG-3'

Other clones with same target sequence:

(none)