Construct: shRNA TRCN0000204677

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_061666.3-699s1c1
DNA Barcode:
GTCCCATTTCGCTGTGGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR4X2 (119764)
Gene Description:
olfactory receptor family 4 subfamily X member 2 (gene/pseudogene)
Transcript:
RefSeq XM_061666.3 (NON-CURRENT)
Match location:
Position 699 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000204677)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCCATTTCGCTGTGGTTAT
Hairpin Sequence:
5'-CCGG-GTCCCATTTCGCTGTGGTTAT-CTCGAG-ATAACCACAGCGAAATGGGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCCATTTCGCTGTGGTTATCTCGAGATAACCACAGCGAAATGGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCCATTTCGCTGTGGTTATCTCGAGATAACCACAGCGAAATGGGAC-3'

Other clones with same target sequence:

(none)