Construct: shRNA TRCN0000204689

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_060535.1-375s1c1
DNA Barcode:
GATCATGAAGGTGGAGGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL18AP4 (127545)
Gene Description:
ribosomal protein L18a pseudogene 4
Transcript:
RefSeq XM_060535.1 (NON-CURRENT)
Match location:
Position 375 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000204689)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCATGAAGGTGGAGGAGAT
Hairpin Sequence:
5'-CCGG-GATCATGAAGGTGGAGGAGAT-CTCGAG-ATCTCCTCCACCTTCATGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCATGAAGGTGGAGGAGATCTCGAGATCTCCTCCACCTTCATGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCATGAAGGTGGAGGAGATCTCGAGATCTCCTCCACCTTCATGATC-3'

Other clones with same target sequence:

(none)