Construct: shRNA TRCN0000204777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_166776.3-330s1c1
DNA Barcode:
GTGTTTCCTCTACAGGGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR10G8 (219869)
Gene Description:
olfactory receptor family 10 subfamily G member 8
Transcript:
RefSeq XM_166776.3 (NON-CURRENT)
Match location:
Position 330 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000204777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTTTCCTCTACAGGGTCAT
Hairpin Sequence:
5'-CCGG-GTGTTTCCTCTACAGGGTCAT-CTCGAG-ATGACCCTGTAGAGGAAACAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTTTCCTCTACAGGGTCATCTCGAGATGACCCTGTAGAGGAAACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTTTCCTCTACAGGGTCATCTCGAGATGACCCTGTAGAGGAAACAC-3'

Other clones with same target sequence:

(none)