Construct: shRNA TRCN0000204875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000627.2-4272s1c3
DNA Barcode:
CCGTTGAATACCGCCTTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LTBP1 (4052)
Gene Description:
latent transforming growth factor beta binding protein 1
Transcript:
RefSeq NM_000627.2 (NON-CURRENT)
Match location:
Position 4272 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000204875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTGAATACCGCCTTGAAT
Hairpin Sequence:
5'-CCGG-CCGTTGAATACCGCCTTGAAT-CTCGAG-ATTCAAGGCGGTATTCAACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTGAATACCGCCTTGAATCTCGAGATTCAAGGCGGTATTCAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTGAATACCGCCTTGAATCTCGAGATTCAAGGCGGTATTCAACGG-3'

Other clones with same target sequence:

  1. TRCN0000169135
  2. TRCN0000299667
  3. TRCN0000053376