Construct: shRNA TRCN0000215317

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029247.3-767s1c1
DNA Barcode:
CTCAACTTTGATGAGTATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930550C14Rik (75311)
Gene Description:
RIKEN cDNA 4930550C14 gene
Transcript:
RefSeq NM_029247.3 (CURRENT)
Match location:
Position 767 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215317)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAACTTTGATGAGTATATT
Hairpin Sequence:
5'-CCGG-CTCAACTTTGATGAGTATATT-CTCGAG-AATATACTCATCAAAGTTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAACTTTGATGAGTATATTCTCGAGAATATACTCATCAAAGTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAACTTTGATGAGTATATTCTCGAGAATATACTCATCAAAGTTGAG-3'

Other clones with same target sequence:

  1. TRCN0000264586