Construct: shRNA TRCN0000215320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172643.5-2334s1c1
DNA Barcode:
CAACATTGCAATGCAACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zbtb41 (226470)
Gene Description:
zinc finger and BTB domain containing 41
Transcript:
RefSeq NM_172643.5 (CURRENT)
Match location:
Position 2334 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACATTGCAATGCAACATTT
Hairpin Sequence:
5'-CCGG-CAACATTGCAATGCAACATTT-CTCGAG-AAATGTTGCATTGCAATGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACATTGCAATGCAACATTTCTCGAGAAATGTTGCATTGCAATGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACATTGCAATGCAACATTTCTCGAGAAATGTTGCATTGCAATGTTG-3'

Other clones with same target sequence:

(none)