Construct: shRNA TRCN0000215327

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001492.2-1370s1c1
DNA Barcode:
CGAAAGTTTCTTCAACGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lca5l (385668)
Gene Description:
Leber congenital amaurosis 5-like
Transcript:
RefSeq NM_001001492.2 (CURRENT)
Match location:
Position 1370 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215327)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGTTTCTTCAACGAAAT
Hairpin Sequence:
5'-CCGG-CGAAAGTTTCTTCAACGAAAT-CTCGAG-ATTTCGTTGAAGAAACTTTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGTTTCTTCAACGAAATCTCGAGATTTCGTTGAAGAAACTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGTTTCTTCAACGAAATCTCGAGATTTCGTTGAAGAAACTTTCG-3'

Other clones with same target sequence:

(none)