Construct: shRNA TRCN0000215364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028932.3-375s1c1
DNA Barcode:
GAAAGACTGTGTGCTCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eaf1 (74427)
Gene Description:
ELL associated factor 1
Transcript:
RefSeq NM_028932.3 (NON-CURRENT)
Match location:
Position 375 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGACTGTGTGCTCATTAT
Hairpin Sequence:
5'-CCGG-GAAAGACTGTGTGCTCATTAT-CTCGAG-ATAATGAGCACACAGTCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGACTGTGTGCTCATTATCTCGAGATAATGAGCACACAGTCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGACTGTGTGCTCATTATCTCGAGATAATGAGCACACAGTCTTTC-3'

Other clones with same target sequence:

  1. TRCN0000241401