Construct: shRNA TRCN0000215374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183167.3-1230s1c1
DNA Barcode:
CTAGAGAGAAACCCTATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
AI987944 (233168)
Gene Description:
expressed sequence AI987944
Transcript:
RefSeq NM_183167.3 (NON-CURRENT)
Match location:
Position 1230 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGAGAGAAACCCTATAAAT
Hairpin Sequence:
5'-CCGG-CTAGAGAGAAACCCTATAAAT-CTCGAG-ATTTATAGGGTTTCTCTCTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGAGAGAAACCCTATAAATCTCGAGATTTATAGGGTTTCTCTCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGAGAGAAACCCTATAAATCTCGAGATTTATAGGGTTTCTCTCTAG-3'

Other clones with same target sequence:

(none)