Construct: shRNA TRCN0000215378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007900.2-1729s1c1
DNA Barcode:
CTCAAGATAAATCAAGCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ect2 (13605)
Gene Description:
ect2 oncogene
Transcript:
RefSeq NM_007900.2 (NON-CURRENT)
Match location:
Position 1729 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAAGATAAATCAAGCTAAA
Hairpin Sequence:
5'-CCGG-CTCAAGATAAATCAAGCTAAA-CTCGAG-TTTAGCTTGATTTATCTTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAAGATAAATCAAGCTAAACTCGAGTTTAGCTTGATTTATCTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAAGATAAATCAAGCTAAACTCGAGTTTAGCTTGATTTATCTTGAG-3'

Other clones with same target sequence:

(none)