Construct: shRNA TRCN0000215382

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177164.3-3840s1c1
DNA Barcode:
CTAGACCCTTCTCTATTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vwc2l (320460)
Gene Description:
von Willebrand factor C domain-containing protein 2-like
Transcript:
RefSeq NM_177164.3 (CURRENT)
Match location:
Position 3840 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215382)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGACCCTTCTCTATTATTA
Hairpin Sequence:
5'-CCGG-CTAGACCCTTCTCTATTATTA-CTCGAG-TAATAATAGAGAAGGGTCTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGACCCTTCTCTATTATTACTCGAGTAATAATAGAGAAGGGTCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGACCCTTCTCTATTATTACTCGAGTAATAATAGAGAAGGGTCTAG-3'

Other clones with same target sequence:

(none)