Construct: shRNA TRCN0000215419

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025793.2-287s1c1
DNA Barcode:
GAAATGTTATTTCGCTGTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr45b (66840)
Gene Description:
WD repeat domain 45B
Transcript:
RefSeq NM_025793.2 (NON-CURRENT)
Match location:
Position 287 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215419)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATGTTATTTCGCTGTAAC
Hairpin Sequence:
5'-CCGG-GAAATGTTATTTCGCTGTAAC-CTCGAG-GTTACAGCGAAATAACATTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATGTTATTTCGCTGTAACCTCGAGGTTACAGCGAAATAACATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATGTTATTTCGCTGTAACCTCGAGGTTACAGCGAAATAACATTTC-3'

Other clones with same target sequence:

(none)