Construct: shRNA TRCN0000215528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178673.3-988s1c1
DNA Barcode:
GAACTAACTCAGGTGATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fstl5 (213262)
Gene Description:
follistatin-like 5
Transcript:
RefSeq NM_178673.3 (NON-CURRENT)
Match location:
Position 988 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTAACTCAGGTGATTAAA
Hairpin Sequence:
5'-CCGG-GAACTAACTCAGGTGATTAAA-CTCGAG-TTTAATCACCTGAGTTAGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTAACTCAGGTGATTAAACTCGAGTTTAATCACCTGAGTTAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTAACTCAGGTGATTAAACTCGAGTTTAATCACCTGAGTTAGTTC-3'

Other clones with same target sequence:

  1. TRCN0000248114