Construct: shRNA TRCN0000215541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028360.2-664s1c1
DNA Barcode:
GGAATTAGCAGAAGATATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc19 (72795)
Gene Description:
tetratricopeptide repeat domain 19
Transcript:
RefSeq NM_028360.2 (CURRENT)
Match location:
Position 664 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAATTAGCAGAAGATATTAT
Hairpin Sequence:
5'-CCGG-GGAATTAGCAGAAGATATTAT-CTCGAG-ATAATATCTTCTGCTAATTCC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAATTAGCAGAAGATATTATCTCGAGATAATATCTTCTGCTAATTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAATTAGCAGAAGATATTATCTCGAGATAATATCTTCTGCTAATTCC-3'

Other clones with same target sequence:

(none)

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