Construct: shRNA TRCN0000215573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032398.1-390s1c1
DNA Barcode:
GATCACCTACATAAACTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plvap (84094)
Gene Description:
plasmalemma vesicle associated protein
Transcript:
RefSeq NM_032398.1 (NON-CURRENT)
Match location:
Position 390 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCACCTACATAAACTATAA
Hairpin Sequence:
5'-CCGG-GATCACCTACATAAACTATAA-CTCGAG-TTATAGTTTATGTAGGTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCACCTACATAAACTATAACTCGAGTTATAGTTTATGTAGGTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCACCTACATAAACTATAACTCGAGTTATAGTTTATGTAGGTGATC-3'

Other clones with same target sequence:

(none)