Construct: shRNA TRCN0000215656

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027134.2-559s1c1
DNA Barcode:
GATTTGATATAGGCCCAATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtfmt (69606)
Gene Description:
mitochondrial methionyl-tRNA formyltransferase
Transcript:
RefSeq NM_027134.2 (NON-CURRENT)
Match location:
Position 559 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215656)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTTGATATAGGCCCAATTC
Hairpin Sequence:
5'-CCGG-GATTTGATATAGGCCCAATTC-CTCGAG-GAATTGGGCCTATATCAAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTTGATATAGGCCCAATTCCTCGAGGAATTGGGCCTATATCAAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTTGATATAGGCCCAATTCCTCGAGGAATTGGGCCTATATCAAATC-3'

Other clones with same target sequence:

(none)