Construct: shRNA TRCN0000215672

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181039.2-2632s1c1
DNA Barcode:
GATGGAACAACTGCTAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrl1 (330814)
Gene Description:
adhesion G protein-coupled receptor L1
Transcript:
RefSeq NM_181039.2 (CURRENT)
Match location:
Position 2632 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215672)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGAACAACTGCTAGATAT
Hairpin Sequence:
5'-CCGG-GATGGAACAACTGCTAGATAT-CTCGAG-ATATCTAGCAGTTGTTCCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGAACAACTGCTAGATATCTCGAGATATCTAGCAGTTGTTCCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGAACAACTGCTAGATATCTCGAGATATCTAGCAGTTGTTCCATC-3'

Other clones with same target sequence:

(none)