Construct: shRNA TRCN0000215675

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008822.1-511s1c1
DNA Barcode:
GAAATTCTCTGTGTACCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pex7 (18634)
Gene Description:
peroxisomal biogenesis factor 7
Transcript:
RefSeq NM_008822.1 (NON-CURRENT)
Match location:
Position 511 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215675)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATTCTCTGTGTACCTTTA
Hairpin Sequence:
5'-CCGG-GAAATTCTCTGTGTACCTTTA-CTCGAG-TAAAGGTACACAGAGAATTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATTCTCTGTGTACCTTTACTCGAGTAAAGGTACACAGAGAATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATTCTCTGTGTACCTTTACTCGAGTAAAGGTACACAGAGAATTTC-3'

Other clones with same target sequence:

(none)