Construct: shRNA TRCN0000215717

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133837.4-782s1c1
DNA Barcode:
CTCATTGACTTTAATCCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdc123 (98828)
Gene Description:
cell division cycle 123
Transcript:
RefSeq NM_133837.4 (CURRENT)
Match location:
Position 782 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215717)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATTGACTTTAATCCATTT
Hairpin Sequence:
5'-CCGG-CTCATTGACTTTAATCCATTT-CTCGAG-AAATGGATTAAAGTCAATGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATTGACTTTAATCCATTTCTCGAGAAATGGATTAAAGTCAATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATTGACTTTAATCCATTTCTCGAGAAATGGATTAAAGTCAATGAG-3'

Other clones with same target sequence:

  1. TRCN0000246990