Construct: shRNA TRCN0000215721

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138672.2-3107s1c1
DNA Barcode:
CAGTTGCTATGGAGATATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stab1 (192187)
Gene Description:
stabilin 1
Transcript:
RefSeq NM_138672.2 (CURRENT)
Match location:
Position 3107 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215721)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTGCTATGGAGATATTAT
Hairpin Sequence:
5'-CCGG-CAGTTGCTATGGAGATATTAT-CTCGAG-ATAATATCTCCATAGCAACTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTGCTATGGAGATATTATCTCGAGATAATATCTCCATAGCAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTGCTATGGAGATATTATCTCGAGATAATATCTCCATAGCAACTG-3'

Other clones with same target sequence:

(none)