Construct: shRNA TRCN0000215812

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027973.3-735s1c1
DNA Barcode:
CATAACGGAGTTGGATGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cenpu (71876)
Gene Description:
centromere protein U
Transcript:
RefSeq NM_027973.3 (CURRENT)
Match location:
Position 735 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215812)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAACGGAGTTGGATGTTAT
Hairpin Sequence:
5'-CCGG-CATAACGGAGTTGGATGTTAT-CTCGAG-ATAACATCCAACTCCGTTATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAACGGAGTTGGATGTTATCTCGAGATAACATCCAACTCCGTTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAACGGAGTTGGATGTTATCTCGAGATAACATCCAACTCCGTTATG-3'

Other clones with same target sequence:

(none)