Construct: shRNA TRCN0000215833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026062.3-1906s1c1
DNA Barcode:
CATCTTAGTATCGTCACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam69a (67266)
Gene Description:
family with sequence similarity 69, member A
Transcript:
RefSeq NM_026062.3 (NON-CURRENT)
Match location:
Position 1906 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTTAGTATCGTCACATTA
Hairpin Sequence:
5'-CCGG-CATCTTAGTATCGTCACATTA-CTCGAG-TAATGTGACGATACTAAGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTTAGTATCGTCACATTACTCGAGTAATGTGACGATACTAAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTTAGTATCGTCACATTACTCGAGTAATGTGACGATACTAAGATG-3'

Other clones with same target sequence:

(none)