Construct: shRNA TRCN0000215845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172722.3-2549s1c1
DNA Barcode:
CTTCTTCGTTGAGACTATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Naa25 (231713)
Gene Description:
N(alpha)-acetyltransferase 25, NatB auxiliary subunit
Transcript:
RefSeq NM_172722.3 (CURRENT)
Match location:
Position 2549 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTTCGTTGAGACTATTTC
Hairpin Sequence:
5'-CCGG-CTTCTTCGTTGAGACTATTTC-CTCGAG-GAAATAGTCTCAACGAAGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTTCGTTGAGACTATTTCCTCGAGGAAATAGTCTCAACGAAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTTCGTTGAGACTATTTCCTCGAGGAAATAGTCTCAACGAAGAAG-3'

Other clones with same target sequence:

(none)