Construct: shRNA TRCN0000215850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177686.3-654s1c1
DNA Barcode:
CAAGGATGTGCTGGAATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clec12a (232413)
Gene Description:
C-type lectin domain family 12, member a
Transcript:
RefSeq NM_177686.3 (NON-CURRENT)
Match location:
Position 654 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGGATGTGCTGGAATTTAT
Hairpin Sequence:
5'-CCGG-CAAGGATGTGCTGGAATTTAT-CTCGAG-ATAAATTCCAGCACATCCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGGATGTGCTGGAATTTATCTCGAGATAAATTCCAGCACATCCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGGATGTGCTGGAATTTATCTCGAGATAAATTCCAGCACATCCTTG-3'

Other clones with same target sequence:

  1. TRCN0000249252