Construct: shRNA TRCN0000215880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019643.3-410s1c1
DNA Barcode:
CCTAGTCTAAAGACAACATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam60a (56306)
Gene Description:
family with sequence similarity 60, member A
Transcript:
RefSeq NM_019643.3 (CURRENT)
Match location:
Position 410 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGTCTAAAGACAACATTG
Hairpin Sequence:
5'-CCGG-CCTAGTCTAAAGACAACATTG-CTCGAG-CAATGTTGTCTTTAGACTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGTCTAAAGACAACATTGCTCGAGCAATGTTGTCTTTAGACTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGTCTAAAGACAACATTGCTCGAGCAATGTTGTCTTTAGACTAGG-3'

Other clones with same target sequence:

(none)