Construct: shRNA TRCN0000215912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152817.4-1979s1c1
DNA Barcode:
CTACTTCCTATATTCGATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc27 (74196)
Gene Description:
tetratricopeptide repeat domain 27
Transcript:
RefSeq NM_152817.4 (CURRENT)
Match location:
Position 1979 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTTCCTATATTCGATTAA
Hairpin Sequence:
5'-CCGG-CTACTTCCTATATTCGATTAA-CTCGAG-TTAATCGAATATAGGAAGTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTTCCTATATTCGATTAACTCGAGTTAATCGAATATAGGAAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTTCCTATATTCGATTAACTCGAGTTAATCGAATATAGGAAGTAG-3'

Other clones with same target sequence:

  1. TRCN0000250609