Construct: shRNA TRCN0000215948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022332.2-1121s1c1
DNA Barcode:
CACCAATGTCTTAGTCTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St7 (64213)
Gene Description:
suppression of tumorigenicity 7
Transcript:
RefSeq NM_022332.2 (NON-CURRENT)
Match location:
Position 1121 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAATGTCTTAGTCTATAT
Hairpin Sequence:
5'-CCGG-CACCAATGTCTTAGTCTATAT-CTCGAG-ATATAGACTAAGACATTGGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAATGTCTTAGTCTATATCTCGAGATATAGACTAAGACATTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAATGTCTTAGTCTATATCTCGAGATATAGACTAAGACATTGGTG-3'

Other clones with same target sequence:

  1. TRCN0000249438